Hotgen Co., Ltd. is a high technology enterprise, specializing in research, manufacture and sale of diagnostic reagents in vitro. Following the trend of precision and instantness in the international medical diagnostic industry.We are one of the few domestic diagnostic reagents enterprises who have self-owed intellectual property rights. We have already owed four invention patents and one PCT patent, and we are the experimental “Patent Engine” enterprise in Beijing also. Our projects include: New hepatitis B virus reproduction marker- Large Protein Pre-S Antigen Diagnostic Kit, New HCC precaution biological marker- AFP-L3 determining reagents, Human Flu. A/B virus rapid test ,etc. We also provide materials for diagnostic reagents manufacture, including gene engineering recombinant syphilis antigens(P15, P17, P47), hepatitis C virus antigen(HCV-Ag) and various McAbs of cancer markers.Projects introductionA new biological marker for HCC-Lens culinaris agglutinin-reactive fraction of AFP(AFP-L3) determining device and diagnostic kit AFP-L3 is one of AFP fractions which is synthesized by malignant cell and comprise special oligosaccharide. Reports at home or abroad have showed that if the ratio of AFP-L3 increased over 10%, the risk of catching HCC had increased highly, and non-malignant diseases hardly synthesize this AFP fraction(AFP-L3).Comparing with ultrasonography(US), AFP-L3 can be detected 9-12 months earlier and shows the remarkable precaution effect to HCC. Our device can separate the AFP-L3 fraction efficiently, the correctness of HCC diagnosis is 81%, and the distinctive diagnosis from benign hepatic disease is over 95%. Therefore AFP-L3 is named a new biological marker for HCC diagnosis. This project owe international PCT patent.